
تعداد نشریات | 31 |
تعداد شمارهها | 357 |
تعداد مقالات | 3,464 |
تعداد مشاهده مقاله | 4,390,706 |
تعداد دریافت فایل اصل مقاله | 5,069,905 |
بررسی صفات بیوشیمیایی والگوی بیان برخی ژنهای کاندید دخیل در تحمل به خشکی دو رقم گندم نان | ||
تولید و ژنتیک گیاهی | ||
دوره 4، شماره 2 - شماره پیاپی 6، اسفند 1402، صفحه 229-242 اصل مقاله (466.97 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22034/plant.2023.139618.1073 | ||
نویسندگان | ||
سیما آبیار1؛ سعید نواب پور* 2؛ نفیسه مهدی نژاد3 | ||
1دانش آموخته دکتری، گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
2استاد، گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
3دانشیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران | ||
چکیده | ||
کمبود آب و به دنبال آن تنش خشکی در گیاهان به عنوان یکی از اولین و مهمترین عوامل اختلال در مکانیسمهای سلولی، عامل تنش اکسیداتیو بوده و تهدید و محدودیت جدی برای محصولات کشاورزی میباشد. در این پژوهش ارزیابی صفات بیوشیمیایی(میزان اکسیداسیون سلولی و کلروفیلa و b) و میزانرونویسی برخی ژنهای آنتیاکسیدانشامل کاتالاز (CAT)، آسکوربات پراکسیداز (APX) و گلوتاتیون ردوکتاز (GR) توسط Real time PCR تحت تاثیر سطوح مختلف تنش خشکی در مزرعه تحقیقاتی دانشکده کشاورزی زابل به صورت آزمایش اسپلیت پلاتدر قالب طرح بلوکهای کامل تصادفیبا سه تکرار بر روی ارقامگندم انجام گرفت. فاکتور اصلی سطوح تنش خشکی شامل 3/0–بار (ظرفیت زراعی بهعنوان تیمار شاهد)، 2- بار و 4- بارو فاکتور فرعی ارقام گندم (کلاته و احسان) بودند. نتایج تجزیه و تحلیل واریانس صفات بیوشیمیایی نشان داد که اثر تنش خشکی برای این صفات و نیز تفاوت بین ارقام معنیدار بود. نتایج بررسی بیان ژنهایCAT، APXو GRدر شرایط اعمال سطوح آبیاری، روند متفاوتی را در ارقام این پژوهش از خود نشان دادند و افزایش بیان همراه با افزایش سطوح تنش خشکیداشتند. این افزایش بیان در رقم کلاته بهترتیببا ( 45/3، 1/10 ، 2/6 برابرنسبتبهشاهد)برای ژن هایCAT، APX ،GRطی تنش 4- بار بیشتر از رقم احسان بود. در این پژوهش رقم کلاته از نظر اکثر صفات بررسی شده در وضعیت مطلوبتری قرار داشت. | ||
کلیدواژهها | ||
گندم؛ تنش خشکی؛ بیان ژن؛ ژن های آنتی اکسیدان | ||
مراجع | ||
Ahmadi, G . H., Siosemarde, A., Sohrabi Y., Jalal kamali., & M. Reza. (2020). Evaluation of response of durum wheat (Triticum durum Desf.) lines to terminal drought stress. Applied Research in Field Crops, 33 (1), 23-43. Talae Ahmad, S., & R. Haddad. (2010). The effect of silicon on the activity of antioxidant enzymes and the content of osmotic regulators in two genotypes of bread wheat under drought stress conditions. Journal of Seedling and Seed Agriculture, 2, 201-225 (In persian). Agarwal, P., Agarwal, P.K. Joshi, A.J. SoporyS.K., & Reddy, M.K. (2010). Over expression of PgDREB2A transcription factor enhances abiotic stress tolerance and activates downstream stress responsive genes. Molecular Biology Reports, 37, 1125 1135 . Ahmad, P. C. A., Jeleel, C. A., Azooz, M. M., & Nabi, G. (2010). Generation of biological activities of Salvia halophila and Salvia virgata from Turkey. Food Chemistry, 108(3), 942-949. Asseng, S., Martre, P., Maiorano, A., Rötter, R. P., O’Leary, G. J., Fitzgerald, G. J., & Ewert, F. (2019). Climate change impact and adaptation for wheat protein. Global Change Biology, 25(1), 155-173. Begara-Morales, J.C., Sanchez-Calvo, B., Chaki, M., Mata-Perez, C., Valderrama, R., Padilla, M.N., Lopez- Jaramillo, J., Luque, F., Corpas, F.J., & Barroso, J.B. (2015). Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation. Journal of Experimental Botany, 66(19), 5983-5996. Bouchemal, K., Bouldjadj, R., Belbekri, M.N., Ykhlef, N., & Djekoun, A. (2017). Differences in antioxidant enzyme activities and oxidative markers in ten wheat (Triticum durum Desf.) genotypes in response to drought, heat and paraquat stress. Archives of Agronomy and Soil Science, 63, 710 -722 . Costa, R.C.L, Lobato A.K.S, Silveira, J.A.G., & Laughinghouse, I.V. (2011). ABAmediatedproline synthesis in cowpea leaves exposed to waterdeficiency and rehydration.Turkish Journal of Agriculture and Forestry, 35, 309-317. Derogar, H., Fakheri, B., Mehdinezhad, N., & Mohammadi, R. (2019). Evaluation of some biochemical traits in cultivars and wild species of wheat under drought stress. Environmental Stresses in Crop Sciences, 12(3), 685- 696. Dhanda, S.S., Sethi G.S., & Behl, R.K. (2004). Indices of drought tolerance inwheat genotypes at early stages of plant growth. Journal Agron Crop Science, 190, 6–12. doi:10.1111/j.1439-037X.2004.00592.x. Du, YY., Wang, P.C., Chen, J., & Song, C.P. (2008). Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana. Journal of Integrative Plant Biology, 50(10): 1318–1326. Edreva A. (2005). Generation and scavenging of reactive oxygen species in chloroplasts: A submolecular approach. Agriculture, Ecosystems and Environment, 106, 119-133. Emadi, N., Balouchi, H.R., & Jahan, B.S. (2012). Effect of drought stress and plant density on yield, yield components and some morphological characters of pinto bean (cv. CO S16) in Yasouj region. Journal of Crop Production, 5 (2), 1 -17. Hassanpour-Lescokelaye, K., Ahmadi, J., Daneshyan, J., & Hatami, S. (2015). Changes in chlorophyll, protein and antioxidant enzymes on durum wheat under drought stress. Journal of Crop Breeding, 7(15), 76-87. (In Persian). Hagege, D., Nouvelot, A., Boucaud, J., & Gaspar, T. (1990). Malondialdehyde titration with thiobarbiturate in plant extracts: avoidance of pigment interference. Phytochemical Analysis, 1, 86-89. Halliwell, B. (1982). The toxic effects of oxygen on plant tissue. In: Oberley, L.W. (ed.), Superoxide dismutase. CRC press Inc. Boca Raton. USA. Herbette, S., Lenne, C., Leblanc, N., Julien, J.L., Drevet, J.R., & Roeckel Drevet, P. (2002). Two GPX-like proteins from Lycopersicon esculentum and Helianthus annuus are antioxidant enzymes with phospholipid hydroperoxide glutathione peroxidase and thioredoxin peroxidase activities. European journal of biochemistry. 269, 2414 – 2420. Hui-Mean, F., Yusop, Z., & Yusof, F. (2018). Drought analysis and water resource availability using standardised precipitation evapotranspiration index. Atmospheric Research, 201, 102-115. Hojati, M., Modarres-Sanavy, A.M.M., Karimi, M., & Ghanati, F. (2011). Responses of growth and antioxidant systems in Carthamus tinctorius L. under water deficit stress. Acta Physiologia Plantarum, 33, 105-112. IPCC, A. (2013). Climate change 2013: the physical science basis. Contribution of working group I to the fifth assessment report of the intergovernmental panel on climate change, 1535. Kaur, G., Singh, H.P., Batish, D.R., & Kumar, R.K. (2012). Growth, photosynthetic activity and oxidative in wheat (Triticum aestivum) after exposure of lead to soil. Journal of Environmental Biology, 33, 265-269 . Kavas, M., Baloğlu, M. C., Akca, O., Köse, F. S., & Gökçay, D. (2013). Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings. Turkish Journal of Biology, 37(4), 491-498 Kazemi, G., Navabpour, S., & Ramezanpour, S.S. (2010). Evaluation of catalase gene expression and morphological traits in two wheat cultivar under salt stress. Modern Genetic Journal, 1, 79-87. (In Persian) Khadka, K., Earl, H. J., Raizada, M. N., & Navabi, A. (2020). A physio-morphological trait-based approach for breeding drought tolerant wheat. Frontiers in plant science, 11, 715. Khan, M.A., Tahir, A., Khurshid, N., Ahmed, M., & Boughanmi, H. (2020). Economic effects of climate changeinduced loss of agricultural production by 2050: a case study of Pakistan. Sustainability, 12(3), 1216 . Kitijima K, Mulkey S, Samaniego M., & Wright S. (2002). Decline of photosynthetic capacity with leaf age and position in two tropical pioneer tree species. American Journal of Botany, 88, 1925-1932. Kovacik, J., Klejdus, B., Babula, P., & Jarosova, M. (2014). Variation of antioxidants and secondary metabolites in nitrogen-deficient barely plants. Journal of Plant Physiology, 171, 260-268. Li, Z., Peng, Y., & Ma, X. (2013). Different response on drought tolerance and postdrought recovery between the small-leafed and the large leafed white clover (Trifolium repens L.) associated with antioxidative enzyme protection and lignin metabolism. Acta Physiologiae Plantarum, 35, 213-222. Lobato, A.K.S, Luz, L.M, Costa Santos, R.C.L, Filho, B.G, Meirelles, A.C.S, OliveiraNeto, C.F, Laughinghouse, H.D, Neto, M.A.M, Alves, G.A.R, Lopes,M.J.S., & Neves, H.K.B. (2009). Si exercises influence on nitrogen components inpepper subjected to water deficit. Res Journal Biolog Science, 4,1048–1055. Lou, L., Li, X., Chen, J., Li, Y., Tang, Y., & Lv J. (2018). Photosynthetic and ascorbate-glutathione metabolism in the flag leaves as compared to spikes under drought stress of winter wheat (Triticum aestivum L.). PLOS One, 13(3), 1-18. Luna, C.M., Pastori, G.M., Driscoll, S., Groten, K., Bernard, S., & Foyer, C. H. (2004). Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. Jornal of Expermental Botany, 56, 417- 423. Moloudi, F., Navabpour, S., Soltanloo, H., Ramezanpour, S.S., & Sadeghipour, H. (2013). Catalase and metallothionein genes expression analysis in wheat cultivars under drought stress condition. Journal of Plant Molecular Breeding, 1(2), 58-64. Movludi, A., Ebadi, A., Jahanbakhsh, S., Davari, M., & Parmoon, G.H. (2014). The effect of water deficit and nitrogen on the antioxidant enzymes activity and quantum yield of barley (Hordeum vulgare L.). Notulae Botanicae Hortical Agrobotanici Cluj-Napoca, 42, 398-404. Navabpour,S., & Najafi,H. (2023). Evaluation of some biochemical traits and expression of two genes from the MYB family in two wheat cultivars under drought stress. Plant genetic research, 9(2),10-22. Ozkur, O., Ozdemir, F., Bor, M. Turkan, I. (2009). Physiochemical and antioxidant responses of the perennial Xerophyte Capparis ovata Desf. to drought. Environmental and Experimental Botany, 66, 487- 492. Pfaff, C. L., Parra, E. J., Bonilla, C. A. R. O. L. I. N. A., Hiester, K., McKeigue, P. M., Kamboh, M. I., & Shriver, M.D. (2001). Population structure in admixed populations: effect of admixture dynamics on the pattern of linkage disequilibrium. The American Journal of Human Genetics, 68(1), 198-207. Pour-Aboughadareh, A.; Etminan, A.; Abdelrahman, M.; Siddique, K.; & Tran, L.S.P. (2020). Assessment of biochemical and physiological parameters of durum wheat genotypes at the seedling stage duting polyethylene glycol-induced water stress. Plant Growth Regulation, 92, 81-93 . Porra, R.J., Thompson, W.A., & Kriedemann, P.E. (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta (BBA)- Bioenergetics, 975, 384-394. Pravalie, R., Sîrodoev, I., Patriche, C., Roșca, B., Piticar, A., Bandoc, G., & Iordache, Ş. 2020. The impact of climate change on agricultural productivity in Romania. A country-scale assessment based on the relationship between climatic water balance and maize yields in recent decades. Agricultural Systems, 179, 102767. Ramirez-Cabral, N.Y., Kumar, L., & Shabani, F. (2017). Global alterations in areas of suitability for maize production from climate change and using a mechanistic species distribution model (CLIMEX). Scientific Reports, 7(1), 1-13. Sandalio, L.M., Dalurzo, H.C., Gomez, M., Romero-Puertas, M.C., & Del Rio, L.A. (2001). Cadmium-induced changes in the growth and oxidative metabolism of pea plants. Journal of Experimental Botany, 52(364), 2115-2126. Simonovicova, A., Barteková, J., Janovová, Ľ., & Luptáková, A. (2010). Behaviour of Fe, Mg and Ca in acid mine drainage and experimental solutions in the presence of Aspergillus niger species isolated from various environment. Nova Biotechnologica et Chimica, 10, 63-69. Tas, S., & Tas, B. (2007). Some physiological responses of drought stress in wheat genotypes with different ploidity in Turkiye. World Journal of Agricultural Sciences, 3(2), 178-183. Tsai, Y-C., Hong, C-Y., Liu, L-F., & Kao, C.H. (2005). Expression of ascorbate peroxidase glutathione reductase in roots of rice seedlings in response to NaCl and H2O2. Journal of plant physiology, 162, 291- 299 Wang, Y., & Frei, M. (2011). Agriculture, Ecosystems and Environment Stressed food – The impact of abiotic environmental stresses on crop quality. Agriculture, Ecosystems & Environment, 141, 271–286. Yang, T., & Poovaiah, B.W. (2002). Hydrogen peroxide homeostasis: Activation of plant catalase by calcium/calmodulin. Proc. Natl. Acad. Sci. U. S. A, 99, 4097-4102. Zamani, S., Naderi, M. R., Soleymani, A., & Nasiri, B. M. (2020). Sunflower (Helianthus annuus L.) biochemical properties and seed components affected by potassium fertilization under drought conditions. Ecotoxicology and Environmental Safety, 190, 110017. Zlatev, Z., & Lidon, F.C. (2012). An overview on drought induced changes in plant growth, water relations and photosynthesis. Emirates Journal of Food and Agriculture, 24, 57-72. | ||
آمار تعداد مشاهده مقاله: 199 تعداد دریافت فایل اصل مقاله: 140 |